Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] SETD7 Rabbit pAb (A9985)

KO/KDValidated

Review (1) Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SETD7 Rabbit pAb (A9985)

Western blot analysis of extracts from normal (control) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 antibody (A9985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review
ABclonal: review for [KO Validated] SETD7 Rabbit pAb(A9985)

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Overview

Product name [KO Validated] SETD7 Rabbit pAb
Catalog No. A9985
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables histone-lysine N-methyltransferase activity and p53 binding activity. Involved in peptidyl-lysine dimethylation and peptidyl-lysine monomethylation. Acts upstream of or within cellular response to DNA damage stimulus and heterochromatin organization. Located in chromosome and nucleolus.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1).
Sequence MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK
Gene ID 80854
Swiss prot Q8WTS6
Synonyms KMT7; SET7; SET9; SET7/9; D7
Calculated MW 41kDa
Observed MW 48kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T
Cellular location Chromosome, Nucleus
Customer validation

WB (Human hepatocellular carcinoma cell lineHomo sapiens, Mus musculus)

IF (Rattus norvegicus)

IHC (Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] SETD7 Rabbit pAb images

ABclonal:Western blot - [KO Validated] SETD7 Rabbit pAb (A9985)}

Western blot - [KO Validated] SETD7 Rabbit pAb (A9985)

Western blot analysis of extracts from normal (control) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 antibody (A9985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SETD7. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SETD7. (Distance between topics and target gene indicate popularity.) SETD7

* Data provided by citexs.com, for reference only.

Publishing research using A9985? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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