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[KO Validated] SETD7 Rabbit pAb (A9985)

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ABclonal:Western blot - [KO Validated] SETD7 Rabbit pAb (A9985)
Review
ABclonal: review for [KO Validated] SETD7 Rabbit pAb(A9985)

Overview

Product name[KO Validated] SETD7 Rabbit pAb
Catalog No.A9985
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1).
SequenceMDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK
Gene ID80854
Swiss protQ8WTS6
SynonymsSETD7; KMT7; SET7; SET7/9; SET9
Calculated MW40kDa
Observed MW48KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationChromosome, Nucleus
Customer validation

(Human hepatocellular carcinoma cell line)

IHC(Rattus norvegicus, Mus musculus)

IF(Rattus norvegicus)

WB(Homo sapiens)

IF(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] SETD7 Rabbit pAb (A9985) }

Western blot - [KO Validated] SETD7 Rabbit pAb (A9985)

Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (A9985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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