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[KO Validated] SETD7 Polyclonal Antibody (A9985)

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ABclonal:Western blot - [KO Validated] SETD7 Polyclonal Antibody (A9985)
ABclonal:Western blot - [KO Validated] SETD7 Polyclonal Antibody (A9985)
Review
ABclonal:Western blot (WB) review for [KO Validated] SETD7 Polyclonal Antibody(A9985)

Overview

Product name[KO Validated] SETD7 Polyclonal Antibody
Catalog No.A9985
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1).
SequenceMDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK
Gene ID80854
Swiss protQ8WTS6
SynonymsSETD7; KMT7; SET7; SET7/9; SET9; histone-lysine N-methyltransferase SETD7
Calculated MW40kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, HepG2, Mouse pancreas, Mouse brain, Rat brain
Cellular locationChromosome, Nucleus
Customer validation

WB(Human hepatocellular carcinoma cell line)

IHC(Rat)

IF(Rat)

ABclonal:Western blot - [KO Validated] SETD7 Polyclonal Antibody (A9985) }

Western blot - [KO Validated] SETD7 Polyclonal Antibody (A9985)

Western blot analysis of extracts of various cell lines, using SETD7 antibody (A9985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] SETD7 Polyclonal Antibody (A9985) }

Western blot - [KO Validated] SETD7 Polyclonal Antibody (A9985)

Western blot analysis of extracts from normal (control) and SETD7 knockout (KO) HeLa cells, using SETD7 antibody (A9985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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