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[KO Validated] SELENBP1 Rabbit pAb (A1222)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)
ABclonal:Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)
ABclonal:Immunohistochemistry - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)
ABclonal:Immunofluorescence - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)
Review
ABclonal: review for [KO Validated] SELENBP1 Rabbit pAb(A1222)

Overview

Product name[KO Validated] SELENBP1 Rabbit pAb
Catalog No.A1222
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).
SequenceSLKDGLIPLEIRFLHNPDAAQGFVGCALSSTIQRFYKNEGGTWSVEKVIQVPPKKVKGWLLPEMPGLITDILLSLDDRFLYFSNWLHGDLRQYDISDPQRPRLTGQLFLGGSIVKGGPVQVLEDEELKSQPEPLVVKGKRVAGGPQMIQLSLDGKRLYITTSLYSAWDKQFYPDLIREGSVMLQVDVDTVKGGLKLNPNFLVDFGKEPLGPALAHELRYPGGDCSSDIWI
Gene ID8991
Swiss protQ13228
SynonymsSELENBP1; HEL-S-134P; LPSB; SBP56; SP56; hSBP
Calculated MW45kDa/52kDa/56kDa
Observed MW55kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Mouse liver, Mouse kideny
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytosol
Customer validation

(Human cells)

    ABclonal:Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (A1222) }

    Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)

    Western blot analysis of extracts of various cell lines, using SELENBP1 antibody (A1222) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    ABclonal:Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (A1222) }

    Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)

    Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody (A1222) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - [KO Validated] SELENBP1 Polyclonal Antibody (A1222) }

    Immunohistochemistry - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)

    Immunohistochemistry of paraffin-embedded human colon using SELENBP1 Antibody (A1222) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - [KO Validated] SELENBP1 Polyclonal Antibody (A1222) }

    Immunofluorescence - [KO Validated] SELENBP1 Polyclonal Antibody (A1222)

    Immunofluorescence analysis of HeLa cells using SELENBP1 antibody (A1222). Blue: DAPI for nuclear staining.

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