Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] SAMHD1 Rabbit pAb (A7794)

Datasheet

ABclonal:Western blot - [KO Validated] SAMHD1 Rabbit pAb (A7794)
ABclonal:Western blot - [KO Validated] SAMHD1 Rabbit pAb (A7794)
ABclonal:Immunohistochemistry - [KO Validated] SAMHD1 Rabbit pAb (A7794)
ABclonal:Immunofluorescence - [KO Validated] SAMHD1 Rabbit pAb (A7794)
ABclonal:Immunoprecipitation - [KO Validated] SAMHD1 Rabbit pAb (A7794)

Overview

Product name[KO Validated] SAMHD1 Rabbit pAb
Catalog No.A7794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).
SequenceTDAFLKADDYIEITGAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEYRNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAEDFIVDVINMDYGMQEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAEQLIRVYCKKVDRKSLYAARQYFVQWCADRNFTKPQDGDVIAPLITPQKKEWNDSTSVQNPTRLREASKSRVQLFKDDPM
Gene ID25939
Swiss protQ9Y3Z3
SynonymsSAMHD1; CHBL2; DCIP; HDDC1; MOP-5; SBBI88
Calculated MW63kDa/68kDa/72kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHepG2, HeLa, MCF-7, Mouse spleen
Cellular locationNucleus
    ABclonal:Western blot - [KO Validated] SAMHD1 Rabbit pAb (A7794) }

    Western blot - [KO Validated] SAMHD1 Rabbit pAb (A7794)

    Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (A7794) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - [KO Validated] SAMHD1 Rabbit pAb (A7794) }

    Western blot - [KO Validated] SAMHD1 Rabbit pAb (A7794)

    Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (A7794) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - [KO Validated] SAMHD1 Rabbit pAb (A7794) }

    Immunohistochemistry - [KO Validated] SAMHD1 Rabbit pAb (A7794)

    Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] SAMHD1 Rabbit pAb (A7794) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - [KO Validated] SAMHD1 Rabbit pAb (A7794) }

    Immunofluorescence - [KO Validated] SAMHD1 Rabbit pAb (A7794)

    Immunofluorescence analysis of A549 cells using SAMHD1 antibody (A7794). Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - [KO Validated] SAMHD1 Rabbit pAb (A7794) }

    Immunoprecipitation - [KO Validated] SAMHD1 Rabbit pAb (A7794)

    Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SAMHD1 antibody (A7794). Western blot was performed from the immunoprecipitate using SAMHD1 antibody (A7794) at a dilition of 1:1000.

    Inquire About This Product

    Submit your question about A7794 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Select size and quantity
    Contact us to order