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[KO Validated] S100A6 Rabbit pAb (A4104)

Datasheet

ABclonal:Western blot - [KO Validated] S100A6 Rabbit pAb (A4104)
ABclonal:Western blot - [KO Validated] S100A6 Rabbit pAb (A4104)
ABclonal:Immunohistochemistry - [KO Validated] S100A6 Rabbit pAb (A4104)
ABclonal:Immunoprecipitation - [KO Validated] S100A6 Rabbit pAb (A4104)

Overview

Product name[KO Validated] S100A6 Rabbit pAb
Catalog No.A4104
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
SequenceMACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQKELTIGSKLQDAEIARLMEDLDRNKDQEVNFQEYVTFLGALALIYNEALKG
Gene ID6277
Swiss protP06703
SynonymsS100A6; 2A9; 5B10; CABP; CACY; PRA
Calculated MW10kDa
Observed MW10KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Mouse lung, Rat lung
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Nucleus envelope, Peripheral membrane protein
ABclonal:Western blot - [KO Validated] S100A6 Rabbit pAb (A4104) }

Western blot - [KO Validated] S100A6 Rabbit pAb (A4104)

Western blot analysis of extracts from normal (control) and S100A6 knockout (KO) HeLa cells, using S100A6 antibody (A4104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] S100A6 Rabbit pAb (A4104) }

Western blot - [KO Validated] S100A6 Rabbit pAb (A4104)

Western blot analysis of extracts of various cell lines, using S100A6 antibody (A4104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KO Validated] S100A6 Rabbit pAb (A4104) }

Immunohistochemistry - [KO Validated] S100A6 Rabbit pAb (A4104)

Immunohistochemistry of paraffin-embedded rat cerebellum using [KO Validated] S100A6 Rabbit pAb (A4104) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] S100A6 Rabbit pAb (A4104) }

Immunoprecipitation - [KO Validated] S100A6 Rabbit pAb (A4104)

Immunoprecipitation analysis of 200ug extracts of U-251MG cells using 3ug S100A6 antibody (A4104). Western blot was performed from the immunoprecipitate using S100A6 antibody (A4104) at a dilition of 1:1000.

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