Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Immunohistochemistry - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Immunohistochemistry - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
ABclonal:Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)
Review
ABclonal: review for [KO Validated] FSP1/S100A4 Rabbit pAb(A1631)

Overview

Product name[KO Validated] FSP1/S100A4 Rabbit pAb
Catalog No.A1631
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human FSP1/S100A4 (NP_002952.1).
SequenceMACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK
Gene ID6275
Swiss protP26447
Synonyms18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; S100A4
Calculated MW11kDa
Observed MW12KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A-549, A375, NIH/3T3, Rat spleen, Rat heart
Cellular location
Customer validation

(mouse cells)

WB(Mus musculus, Homo sapiens)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Western blot analysis of extracts from normal (control) and FSP1/S100A4 knockout (KO) HeLa cells, using FSP1/S100A4 antibody (A1631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Western blot analysis of extracts of various cell lines, using FSP1/S100A4 antibody (A1631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Western blot - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Western blot analysis of extracts of various cell lines, using FSP1/S100A4 antibody (A1631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Immunohistochemistry - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Immunohistochemistry - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Immunofluorescence analysis of HeLa cells using FSP1/S100A4 antibody (A1631) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Immunofluorescence analysis of PC12 cells using FSP1/S100A4 antibody (A1631) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631) }

Immunofluorescence - [KO Validated] FSP1/S100A4 Rabbit pAb (A1631)

Immunofluorescence analysis of RAW264.7 cells using FSP1/S100A4 antibody (A1631) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A1631

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A1631 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1631? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order