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[KO Validated] S100A11 Rabbit pAb (A5486)

Datasheet

ABclonal:Western blot - [KO Validated] S100A11 Rabbit pAb (A5486)
ABclonal:Western blot - [KO Validated] S100A11 Rabbit pAb (A5486)
ABclonal:Immunohistochemistry - [KO Validated] S100A11 Rabbit pAb (A5486)
ABclonal:Immunofluorescence - [KO Validated] S100A11 Rabbit pAb (A5486)
ABclonal:Immunoprecipitation - [KO Validated] S100A11 Rabbit pAb (A5486)

Overview

Product name[KO Validated] S100A11 Rabbit pAb
Catalog No.A5486
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).
SequenceMAKISSPTETERCIESLIAVFQKYAGKDGYNYTLSKTEFLSFMNTELAAFTKNQKDPGVLDRMMKKLDTNSDGQLDFSEFLNLIGGLAMACHDSFLKAVPSQKRT
Gene ID6282
Swiss protP31949
SynonymsS100A11; HEL-S-43; MLN70; S100C
Calculated MW11kDa
Observed MW12kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesBxPC-3, BT-474, LOVO, 22Rv1, SKOV3, THP-1
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - [KO Validated] S100A11 Rabbit pAb (A5486) }

Western blot - [KO Validated] S100A11 Rabbit pAb (A5486)

Western blot analysis of extracts of various cell lines, using S100A11 antibody (A5486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] S100A11 Rabbit pAb (A5486) }

Western blot - [KO Validated] S100A11 Rabbit pAb (A5486)

Western blot analysis of extracts from normal (control) and S100A11 knockout (KO) HeLa cells, using S100A11 antibody (A5486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] S100A11 Rabbit pAb (A5486) }

Immunohistochemistry - [KO Validated] S100A11 Rabbit pAb (A5486)

Immunohistochemistry of paraffin-embedded human kidney using S100A11 antibody (A5486) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] S100A11 Rabbit pAb (A5486) }

Immunofluorescence - [KO Validated] S100A11 Rabbit pAb (A5486)

Immunofluorescence analysis of U2OS cells using S100A11 antibody (A5486). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] S100A11 Rabbit pAb (A5486) }

Immunoprecipitation - [KO Validated] S100A11 Rabbit pAb (A5486)

Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug S100A11 antibody (A5486). Western blot was performed from the immunoprecipitate using S100A11 antibody (A5486) at a dilition of 1:1000.

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