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[KO Validated] ROCK1 Rabbit pAb (A1008)

Datasheet

ABclonal:Western blot - [KO Validated] ROCK1 Rabbit pAb (A1008)
ABclonal:Western blot - [KO Validated] ROCK1 Rabbit pAb (A1008)
ABclonal:Immunohistochemistry - [KO Validated] ROCK1 Rabbit pAb (A1008)
ABclonal:Immunoprecipitation - [KO Validated] ROCK1 Rabbit pAb (A1008)

Overview

Product name[KO Validated] ROCK1 Rabbit pAb
Catalog No.A1008
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
SequenceYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN
Gene ID6093
Swiss protQ13464
SynonymsROCK1; P160ROCK; ROCK-I
Calculated MW158kDa
Observed MW158kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, U-251MG, Jurkat, BT-474
Cellular locationCell membrane, Cell projection, Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, bleb, centriole, centrosome, cytoskeleton, lamellipodium, microtubule organizing center, ruffle
ABclonal:Western blot - [KO Validated] ROCK1 Rabbit pAb (A1008) }

Western blot - [KO Validated] ROCK1 Rabbit pAb (A1008)

Western blot analysis of extracts of various cell lines, using ROCK1 antibody (A1008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] ROCK1 Rabbit pAb (A1008) }

Western blot - [KO Validated] ROCK1 Rabbit pAb (A1008)

Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody (A1008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] ROCK1 Rabbit pAb (A1008) }

Immunohistochemistry - [KO Validated] ROCK1 Rabbit pAb (A1008)

Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody (A1008) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] ROCK1 Rabbit pAb (A1008) }

Immunoprecipitation - [KO Validated] ROCK1 Rabbit pAb (A1008)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody (A1008). Western blot was performed from the immunoprecipitate using ROCK1 antibody (A1008) at a dilition of 1:1000.

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