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[KO Validated] RHOC Rabbit pAb (A1062)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] RHOC Polyclonal Antibody (A1062)
ABclonal:Western blot - [KO Validated] RHOC Polyclonal Antibody (A1062)
ABclonal:Immunofluorescence - [KO Validated] RHOC Polyclonal Antibody (A1062)

Overview

Product name[KO Validated] RHOC Rabbit pAb
Catalog No.A1062
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
SequenceMAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL
Gene ID389
Swiss protP08134
SynonymsRHOC; ARH9; ARHC; H9; RHOH9
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU2OS, Jurkat, A-549, PC-12
Cellular locationCell membrane, Cleavage furrow, Cytoplasmic side, Lipid-anchor
Customer validation

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] RHOC Polyclonal Antibody (A1062) }

Western blot - [KO Validated] RHOC Polyclonal Antibody (A1062)

Western blot analysis of extracts of various cell lines, using RHOC antibody (A1062).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] RHOC Polyclonal Antibody (A1062) }

Western blot - [KO Validated] RHOC Polyclonal Antibody (A1062)

Western blot analysis of extracts from normal (control) and RHOC knockout (KO) 293T cells, using RHOC antibody (A1062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.
ABclonal:Immunofluorescence - [KO Validated] RHOC Polyclonal Antibody (A1062) }

Immunofluorescence - [KO Validated] RHOC Polyclonal Antibody (A1062)

Immunofluorescence analysis of HeLa cells using RHOC antibody (A1062). Blue: DAPI for nuclear staining.

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