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[KO Validated] RALBP1 Rabbit pAb (A1140)

Datasheet

ABclonal:Western blot - [KO Validated] RALBP1 Rabbit pAb (A1140)
ABclonal:Western blot - [KO Validated] RALBP1 Rabbit pAb (A1140)
ABclonal:Immunohistochemistry - [KO Validated] RALBP1 Rabbit pAb (A1140)
ABclonal:Immunohistochemistry - [KO Validated] RALBP1 Rabbit pAb (A1140)

Overview

Product name[KO Validated] RALBP1 Rabbit pAb
Catalog No.A1140
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1).
SequenceAGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAKEQPKAGKEPAKPSPSRDRKETSI
Gene ID10928
Swiss protQ15311
SynonymsRALBP1; RIP1; RLIP1; RLIP76
Calculated MW76kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-673, Mouse spleen, Mouse lung
Cellular locationMembrane, Peripheral membrane protein
ABclonal:Western blot - [KO Validated] RALBP1 Rabbit pAb (A1140) }

Western blot - [KO Validated] RALBP1 Rabbit pAb (A1140)

Western blot analysis of extracts of various cell lines, using RALBP1 antibody (A1140) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] RALBP1 Rabbit pAb (A1140) }

Western blot - [KO Validated] RALBP1 Rabbit pAb (A1140)

Western blot analysis of extracts from normal (control) and RALBP1 knockout (KO) HeLa cells, using RALBP1 antibody (A1140) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] RALBP1 Rabbit pAb (A1140) }

Immunohistochemistry - [KO Validated] RALBP1 Rabbit pAb (A1140)

Immunohistochemistry of paraffin-embedded rat spleen using RALBP1 antibody (A1140) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] RALBP1 Rabbit pAb (A1140) }

Immunohistochemistry - [KO Validated] RALBP1 Rabbit pAb (A1140)

Immunohistochemistry of paraffin-embedded rat heart using RALBP1 antibody (A1140) at dilution of 1:100 (40x lens).

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