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[KO Validated] RAB5A Rabbit pAb (A1180)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] RAB5A Rabbit pAb (A1180)
ABclonal:Western blot - [KO Validated] RAB5A Rabbit pAb (A1180)
ABclonal:Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180)
ABclonal:Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180)
ABclonal:Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180)

Overview

Product name[KO Validated] RAB5A Rabbit pAb
Catalog No.A1180
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
RAB5A (RAB5A, member RAS oncogene family) is a protein-coding gene. Diseases associated with RAB5A include borna disease, and venezuelan equine encephalitis. GO annotations related to this gene include GDP binding and GTP binding. An important paralog of this gene is RAB20.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5A (NP_004153.2).
SequenceMASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN
Gene ID5868
Swiss protP20339
SynonymsRAB5A; RAB5
Calculated MW22kDa/23kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, PC-12, Mouse brain
Cellular locationCell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Early endosome membrane, Lipid-anchor, Melanosome, Membrane, cytosol, ruffle
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] RAB5A Rabbit pAb (A1180) }

Western blot - [KO Validated] RAB5A Rabbit pAb (A1180)

Western blot analysis of extracts of various cell lines, using RAB5A antibody (A1180) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] RAB5A Rabbit pAb (A1180) }

Western blot - [KO Validated] RAB5A Rabbit pAb (A1180)

Western blot analysis of extracts from normal (control) and RAB5A knockout (KO) HeLa cells, using RAB5A antibody (A1180) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180) }

Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180)

Immunofluorescence analysis of C6 cells using [KO Validated] RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180) }

Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180) }

Immunofluorescence - [KO Validated] RAB5A Rabbit pAb (A1180)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] RAB5A Rabbit pAb (A1180) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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