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[KO Validated] PTPN1 Polyclonal Antibody (A1590)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] PTPN1 Polyclonal Antibody (A1590)
ABclonal:Western blot - [KO Validated] PTPN1 Polyclonal Antibody (A1590)
ABclonal:Immunohistochemistry - [KO Validated] PTPN1 Polyclonal Antibody (A1590)
ABclonal:Immunohistochemistry - [KO Validated] PTPN1 Polyclonal Antibody (A1590)
ABclonal:Immunofluorescence - [KO Validated] PTPN1 Polyclonal Antibody (A1590)
Review
ABclonal: review for [KO Validated] PTPN1 Polyclonal Antibody(A1590)
Review
ABclonal: review for [KO Validated] PTPN1 Polyclonal Antibody(A1590)

Overview

Product name[KO Validated] PTPN1 Polyclonal Antibody
Catalog No.A1590
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
SequenceIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT
Gene ID5770
Swiss protP18031
SynonymsPTPN1; PTP1B; tyrosine-protein phosphatase non-receptor type 1
Calculated MW49kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, SKOV3, U-251MG, Mouse brain, Rat brain
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Peripheral membrane protein
Customer validation

(, )

WB(Mouse)

ABclonal:Western blot - [KO Validated] PTPN1 Polyclonal Antibody (A1590) }

Western blot - [KO Validated] PTPN1 Polyclonal Antibody (A1590)

Western blot analysis of extracts of various cell lines, using PTPN1 antibody (A1590) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] PTPN1 Polyclonal Antibody (A1590) }

Western blot - [KO Validated] PTPN1 Polyclonal Antibody (A1590)

Western blot analysis of extracts from normal (control) and PTPN1 knockout (KO) HeLa cells, using PTPN1 antibody (A1590) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] PTPN1 Polyclonal Antibody (A1590) }

Immunohistochemistry - [KO Validated] PTPN1 Polyclonal Antibody (A1590)

Immunohistochemistry of paraffin-embedded rat brain using PTPN1 antibody (A1590) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] PTPN1 Polyclonal Antibody (A1590) }

Immunohistochemistry - [KO Validated] PTPN1 Polyclonal Antibody (A1590)

Immunohistochemistry of paraffin-embedded mouse brain using PTPN1 antibody (A1590) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] PTPN1 Polyclonal Antibody (A1590) }

Immunofluorescence - [KO Validated] PTPN1 Polyclonal Antibody (A1590)

Immunofluorescence analysis of MCF-7 cells using PTPN1 antibody (A1590). Blue: DAPI for nuclear staining.

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