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[KO Validated] PTPN11 Rabbit pAb (A12486)

Datasheet

ABclonal:Western blot - [KO Validated] PTPN11 Rabbit pAb (A12486)
ABclonal:Western blot - [KO Validated] PTPN11 Rabbit pAb (A12486)
ABclonal:Immunofluorescence - [KO Validated] PTPN11 Rabbit pAb (A12486)
ABclonal:Immunoprecipitation - [KO Validated] PTPN11 Rabbit pAb (A12486)

Overview

Product name[KO Validated] PTPN11 Rabbit pAb
Catalog No.A12486
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human PTPN11 (NP_002825.3).
SequenceMTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCS
Gene ID5781
Swiss protQ06124
SynonymsBPTP3; CFC; JMML; METCDS; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2; PTPN11
Calculated MW52kDa/68kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, Jurkat, A-431, MCF-7, Mouse brain, Rat brain, Rat lung
Cellular locationCytoplasm
ABclonal:Western blot - [KO Validated] PTPN11 Rabbit pAb (A12486) }

Western blot - [KO Validated] PTPN11 Rabbit pAb (A12486)

Western blot analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using PTPN11 antibody (A12486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] PTPN11 Rabbit pAb (A12486) }

Western blot - [KO Validated] PTPN11 Rabbit pAb (A12486)

Western blot analysis of extracts of various cell lines, using PTPN11 antibody (A12486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - [KO Validated] PTPN11 Rabbit pAb (A12486) }

Immunofluorescence - [KO Validated] PTPN11 Rabbit pAb (A12486)

Immunofluorescence analysis of C6 cells using PTPN11 antibody (A12486) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] PTPN11 Rabbit pAb (A12486) }

Immunoprecipitation - [KO Validated] PTPN11 Rabbit pAb (A12486)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug PTPN11 antibody (A12486). Western blot was performed from the immunoprecipitate using PTPN11 antibody (A12486) at a dilition of 1:2000.

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