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[KO Validated] PRKAR2A Rabbit pAb (A1531)

Datasheet

ABclonal:Western blot - [KO Validated] PRKAR2A Rabbit pAb (A1531)
ABclonal:Western blot - [KO Validated] PRKAR2A Rabbit pAb (A1531)
ABclonal:Immunofluorescence - [KO Validated] PRKAR2A Rabbit pAb (A1531)

Overview

Product name[KO Validated] PRKAR2A Rabbit pAb
Catalog No.A1531
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).
SequenceMSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ
Gene ID5576
Swiss protP13861
SynonymsPRKAR2A; PKR2; PRKAR2
Calculated MW43kDa/45kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, Jurkat, SW480, LO2, Mouse lung, Rat skeletal muscle
Cellular locationCell membrane, Cytoplasm
ABclonal:Western blot - [KO Validated] PRKAR2A Rabbit pAb (A1531) }

Western blot - [KO Validated] PRKAR2A Rabbit pAb (A1531)

Western blot analysis of extracts of various cell lines, using PRKAR2A antibody (A1531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] PRKAR2A Rabbit pAb (A1531) }

Western blot - [KO Validated] PRKAR2A Rabbit pAb (A1531)

Western blot analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using PRKAR2A antibody (A1531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] PRKAR2A Rabbit pAb (A1531) }

Immunofluorescence - [KO Validated] PRKAR2A Rabbit pAb (A1531)

Immunofluorescence analysis of L929 cells using [KO Validated] PRKAR2A Rabbit pAb (A1531) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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