Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

KO/KDValidated

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of extracts from normal (control) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using PKA RIIα (PRKAR2A)/PKR2 antibody (A1531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of extracts of various cell lines, using PKA RIIα (PRKAR2A)/PKR2 antibody (A1531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Immunofluorescence analysis of L929 cells using [KO Validated] PKA RIIα (PRKAR2A)/PKR2 Rabbit pAb (A1531) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb
Catalog No. A1531
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RIIα (PRKAR2A)/PKR2 (NP_004148.1).
Sequence MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ
Gene ID 5576
Swiss prot P13861
Synonyms PKR2; PRKAR2; A)
Calculated MW 46kDa
Observed MW 46kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, Jurkat, SW480, LO2, Mouse lung, Rat skeletal muscle
Cellular location Cell membrane, Cytoplasm
Customer validation

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb images

ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)}

Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of extracts from normal (control) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using PKA RIIα (PRKAR2A)/PKR2 antibody (A1531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)}

Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of extracts of various cell lines, using PKA RIIα (PRKAR2A)/PKR2 antibody (A1531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)}

Immunofluorescence - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Immunofluorescence analysis of L929 cells using [KO Validated] PKA RIIα (PRKAR2A)/PKR2 Rabbit pAb (A1531) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKAR2A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKAR2A. (Distance between topics and target gene indicate popularity.) PRKAR2A

* Data provided by citexs.com, for reference only.

Publishing research using A1531? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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