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[KO Validated] AMPKβ1 Rabbit pAb (A12491)

Datasheet

ABclonal:Western blot - [KO Validated] AMPKβ1 Rabbit pAb (A12491)
ABclonal:Western blot - [KO Validated] AMPKβ1 Rabbit pAb (A12491)
ABclonal:Immunohistochemistry - [KO Validated] AMPKβ1 Rabbit pAb (A12491)
ABclonal:Immunofluorescence - [KO Validated] AMPKβ1 Rabbit pAb (A12491)

Overview

Product name[KO Validated] AMPKβ1 Rabbit pAb
Catalog No.A12491
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPKβ1 (NP_006244.2).
SequenceMGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPT
Gene ID5564
Swiss protQ9Y478
SynonymsPRKAB1; AMPK; HAMPKb
Calculated MW30kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, A-549, HeLa, Jurkat, HepG2
Cellular location
ABclonal:Western blot - [KO Validated] AMPKβ1 Rabbit pAb (A12491) }

Western blot - [KO Validated] AMPKβ1 Rabbit pAb (A12491)

Western blot analysis of extracts of various cell lines, using AMPKβ1 antibody (A12491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] AMPKβ1 Rabbit pAb (A12491) }

Western blot - [KO Validated] AMPKβ1 Rabbit pAb (A12491)

Western blot analysis of extracts from normal (control) and AMPKβ1 knockout (KO) HeLa cells, using AMPKβ1 antibody (A12491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] AMPKβ1 Rabbit pAb (A12491) }

Immunohistochemistry - [KO Validated] AMPKβ1 Rabbit pAb (A12491)

Immunohistochemistry of paraffin-embedded human esophagus using AMPKβ1 antibody (A12491) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] AMPKβ1 Rabbit pAb (A12491) }

Immunofluorescence - [KO Validated] AMPKβ1 Rabbit pAb (A12491)

Immunofluorescence analysis of NIH-3T3 cells using AMPKβ1 Polyclonal Antibody (A12491) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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