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[KO Validated] PRDX4 Rabbit pAb (A1486)

Datasheet

ABclonal:Western blot - [KO Validated] PRDX4 Polyclonal Antibody (A1486)
ABclonal:Western blot - [KO Validated] PRDX4 Polyclonal Antibody (A1486)

Overview

Product name[KO Validated] PRDX4 Rabbit pAb
Catalog No.A1486
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human PRDX4 (NP_006397.1).
SequenceWETEERPRTREEECHFYAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVIDGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRLEEFRSINTEVVACSVDSQFTHLAWINTPRRQGGLGPIRIPLLSDLTHQISKDYGVYLEDSGHTLRGLFIIDDKGILRQITLNDLPVGRSVDETLRLVQAFQYTDKHGEVCPAGWKPGSETIIPDPAGKLKYFDKLN
Gene ID10549
Swiss protQ13162
SynonymsPRDX4; AOE37-2; AOE372; HEL-S-97n; PRX-4
Calculated MW30kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesH460, PC-3, HeLa, MCF-7, Mouse testis
Cellular locationCytoplasm, Secreted
    ABclonal:Western blot - [KO Validated] PRDX4 Polyclonal Antibody (A1486) }

    Western blot - [KO Validated] PRDX4 Polyclonal Antibody (A1486)

    Western blot analysis of extracts of various cell lines, using PRDX4 antibody (A1486) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Western blot - [KO Validated] PRDX4 Polyclonal Antibody (A1486) }

    Western blot - [KO Validated] PRDX4 Polyclonal Antibody (A1486)

    Western blot analysis of extracts from normal (control) and PRDX4 knockout (KO) 293T cells, using PRDX4 antibody (A1486) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

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