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[KO Validated] PRDX2 Rabbit pAb (A1919)

Datasheet

ABclonal:Western blot - [KO Validated] PRDX2 Polyclonal Antibody (A1919)
ABclonal:Western blot - [KO Validated] PRDX2 Polyclonal Antibody (A1919)
ABclonal:Immunofluorescence - [KO Validated] PRDX2 Polyclonal Antibody (A1919)

Overview

Product name[KO Validated] PRDX2 Rabbit pAb
Catalog No.A1919
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human PRDX2 (NP_859428.1).
SequenceMASGNARIGKPAPDFKATAVVDGAFKEVKLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSNRAEDFRKLGCEVLGVSVDSQFTHLAWYEQGPKREVAAKLTPSGPSSVASWPLLNLWNLRFPIVKIMETLPPKSLRMMTVISI
Gene ID7001
Swiss protP32119
SynonymsPRDX2; HEL-S-2a; NKEF-B; NKEFB; PRP; PRX2; PRXII; PTX1; TDPX1; TPX1; TSA
Calculated MW15kDa/21kDa
Observed MW20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesSW480, 22Rv1, HepG2, K-562, BT-474, Mouse brain, Mouse kidney
Cellular locationCytoplasm
ABclonal:Western blot - [KO Validated] PRDX2 Polyclonal Antibody (A1919) }

Western blot - [KO Validated] PRDX2 Polyclonal Antibody (A1919)

Western blot analysis of extracts of various cell lines, using PRDX2 antibody (A1919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] PRDX2 Polyclonal Antibody (A1919) }

Western blot - [KO Validated] PRDX2 Polyclonal Antibody (A1919)

Western blot analysis of extracts from normal (control) and PRDX2 knockout (KO) 293T cells, using PRDX2 antibody (A1919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] PRDX2 Polyclonal Antibody (A1919) }

Immunofluorescence - [KO Validated] PRDX2 Polyclonal Antibody (A1919)

Immunofluorescence analysis of HeLa cells using PRDX2 antibody (A1919).

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