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[KO Validated] PPP1CC Rabbit pAb (A4035)

Datasheet

ABclonal:Western blot - [KO Validated] PPP1CC Polyclonal Antibody (A4035)
ABclonal:Western blot - [KO Validated] PPP1CC Polyclonal Antibody (A4035)
ABclonal:Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035)
ABclonal:Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035)
ABclonal:Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035)

Overview

Product name[KO Validated] PPP1CC Rabbit pAb
Catalog No.A4035
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
SequenceMADLDKLNIDSIIQRLLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKKKPNATRPVTPPRGMITKQAKK
Gene ID5501
Swiss protP36873
SynonymsPPP1CC; PP-1G; PP1C; PPP1G
Calculated MW36kDa/38kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
ChIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, HL-60, Mouse thymus, Mouse spleen, Rat brain
Cellular locationChromosome, Cleavage furrow, Cytoplasm, Midbody, Mitochondrion, Nucleus, Nucleus speckle, centromere, kinetochore, nucleolus, nucleoplasm
ABclonal:Western blot - [KO Validated] PPP1CC Polyclonal Antibody (A4035) }

Western blot - [KO Validated] PPP1CC Polyclonal Antibody (A4035)

Western blot analysis of extracts of various cell lines, using PPP1CC antibody (A4035) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] PPP1CC Polyclonal Antibody (A4035) }

Western blot - [KO Validated] PPP1CC Polyclonal Antibody (A4035)

Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (A4035) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035) }

Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035)

Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (A4035) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035) }

Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035)

Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (A4035) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035) }

Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (A4035)

Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (A4035) at dilution of 1:100 (40x lens).

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