Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] CYPOR Rabbit pAb (A8142)

Datasheet

ABclonal:Western blot - [KO Validated] CYPOR Rabbit pAb (A8142)
ABclonal:Western blot - [KO Validated] CYPOR Rabbit pAb (A8142)
ABclonal:Immunohistochemistry - [KO Validated] CYPOR Rabbit pAb (A8142)
ABclonal:Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142)
ABclonal:Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142)
ABclonal:Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142)

Overview

Product name[KO Validated] CYPOR Rabbit pAb
Catalog No.A8142
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).
SequenceMINMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEESSIRQYEL
Gene ID5447
Swiss protP16435
SynonymsPOR; CPR; CYPOR; P450R
Calculated MW76kDa
Observed MW77kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, HeLa, MCF-7, 293T, Mouse liver, Mouse skin, Mouse kidney, Rat brain
Cellular locationEndoplasmic reticulum membrane, Peripheral membrane protein
ABclonal:Western blot - [KO Validated] CYPOR Rabbit pAb (A8142) }

Western blot - [KO Validated] CYPOR Rabbit pAb (A8142)

Western blot analysis of extracts of various cell lines, using CYPOR antibody (A8142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] CYPOR Rabbit pAb (A8142) }

Western blot - [KO Validated] CYPOR Rabbit pAb (A8142)

Western blot analysis of extracts from normal (control) and CYPOR knockout (KO) HeLa cells, using CYPOR antibody (A8142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] CYPOR Rabbit pAb (A8142) }

Immunohistochemistry - [KO Validated] CYPOR Rabbit pAb (A8142)

Immunohistochemistry of paraffin-embedded human esophagus using CYPOR antibody (A8142) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142) }

Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142)

Immunofluorescence analysis of C6 cells using CYPOR Polyclonal Antibody (A8142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142) }

Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142)

Immunofluorescence analysis of HeLa cells using CYPOR Polyclonal Antibody (A8142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142) }

Immunofluorescence - [KO Validated] CYPOR Rabbit pAb (A8142)

Immunofluorescence analysis of NIH-3T3 cells using CYPOR Polyclonal Antibody (A8142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A8142 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order