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[KO Validated] POLE3 Rabbit pAb (A6469)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] POLE3 Rabbit pAb (A6469)
ABclonal:Western blot - [KO Validated] POLE3 Rabbit pAb (A6469)
ABclonal:Immunofluorescence - [KO Validated] POLE3 Rabbit pAb (A6469)
ABclonal:Immunofluorescence - [KO Validated] POLE3 Rabbit pAb (A6469)

Overview

Product name[KO Validated] POLE3 Rabbit pAb
Catalog No.A6469
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).
SequenceMAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN
Gene ID54107
Swiss protQ9NRF9
SynonymsPOLE3; CHARAC17; CHRAC17; YBL1; p17
Calculated MW16kDa
Observed MW20-25kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHepG2, Mouse testis, Mouse bone marrow, Mouse spleen, 293T
Cellular locationNucleus
Customer validation

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] POLE3 Rabbit pAb (A6469) }

Western blot - [KO Validated] POLE3 Rabbit pAb (A6469)

Western blot analysis of extracts of various cell lines, using POLE3 antibody (A6469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] POLE3 Rabbit pAb (A6469) }

Western blot - [KO Validated] POLE3 Rabbit pAb (A6469)

Western blot analysis of extracts from normal (control) and [KO Validated] POLE3 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit pAb antibody (A6469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - [KO Validated] POLE3 Rabbit pAb (A6469) }

Immunofluorescence - [KO Validated] POLE3 Rabbit pAb (A6469)

Immunofluorescence analysis of U2OS cells using POLE3 antibody (A6469).
ABclonal:Immunofluorescence - [KO Validated] POLE3 Rabbit pAb (A6469) }

Immunofluorescence - [KO Validated] POLE3 Rabbit pAb (A6469)

Immunofluorescence analysis of U2OS cells using POLE3 antibody (A6469). Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

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