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[KO Validated] POLB Polyclonal Antibody (A1681)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] POLB Polyclonal Antibody (A1681)
ABclonal:Western blot - [KO Validated] POLB Polyclonal Antibody (A1681)
ABclonal:Immunofluorescence - [KO Validated] POLB Polyclonal Antibody (A1681)
Review
ABclonal:Immunofluorescence (IF) review for [KO Validated] POLB Polyclonal Antibody(A1681)

Overview

Product name[KO Validated] POLB Polyclonal Antibody
Catalog No.A1681
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).
SequenceMSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
Gene ID5423
Swiss protP06746
SynonymsPOLB; DNA polymerase beta
Calculated MW38kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:1000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, Mouse skeletal muscle
Cellular locationCytoplasm, Nucleus
Customer validation

IF(Human renal epithelial cell line)

WB(Human)

ABclonal:Western blot - [KO Validated] POLB Polyclonal Antibody (A1681) }

Western blot - [KO Validated] POLB Polyclonal Antibody (A1681)

Western blot analysis of extracts of various cell lines, using POLB antibody (A1681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] POLB Polyclonal Antibody (A1681) }

Western blot - [KO Validated] POLB Polyclonal Antibody (A1681)

Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibody (A1681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - [KO Validated] POLB Polyclonal Antibody (A1681) }

Immunofluorescence - [KO Validated] POLB Polyclonal Antibody (A1681)

Immunofluorescence analysis of U2OS cells using POLB antibody (A1681). Blue: DAPI for nuclear staining.

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