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[KO Validated] PML Rabbit pAb (A1184)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] PML Rabbit pAb (A1184)
ABclonal:Western blot - [KO Validated] PML Rabbit pAb (A1184)
ABclonal:Immunohistochemistry - [KO Validated] PML Rabbit pAb (A1184)
ABclonal:Immunofluorescence - [KO Validated] PML Rabbit pAb (A1184)

Overview

Product name[KO Validated] PML Rabbit pAb
Catalog No.A1184
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).
SequenceAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSCMEPMETAEPQSSPAHSSPAHSSPAHSSPVQSLLRAQGASSLPCGTYHPPAWPPHQPAEQAATPDAEPHSEPPDHQER
Gene ID5371
Swiss protP29590
SynonymsMYL; PP8675; RNF71; TRIM19; PML
Calculated MW47-48kDa/62-97kDa
Observed MW70-130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesDU145
Cellular locationCytoplasm, Cytoplasmic side, Early endosome membrane, Endoplasmic reticulum membrane, Nucleus, PML body, Peripheral membrane protein, nucleolus, nucleoplasm
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] PML Rabbit pAb (A1184) }

Western blot - [KO Validated] PML Rabbit pAb (A1184)

Western blot analysis of extracts of various cell lines, using PML antibody (A1184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] PML Rabbit pAb (A1184) }

Western blot - [KO Validated] PML Rabbit pAb (A1184)

Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (A1184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] PML Rabbit pAb (A1184) }

Immunohistochemistry - [KO Validated] PML Rabbit pAb (A1184)

Immunohistochemistry of paraffin-embedded human appendix using PML antibody (A1184) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] PML Rabbit pAb (A1184) }

Immunofluorescence - [KO Validated] PML Rabbit pAb (A1184)

Immunofluorescence analysis of HeLa cells using PML antibody (A1184). Blue: DAPI for nuclear staining.

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