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[KO Validated] PFKP Rabbit pAb (A7916)

Datasheet

ABclonal:Western blot - [KO Validated] PFKP Rabbit pAb (A7916)
ABclonal:Western blot - [KO Validated] PFKP Rabbit pAb (A7916)
ABclonal:Immunohistochemistry - [KO Validated] PFKP Rabbit pAb (A7916)
ABclonal:Immunohistochemistry - [KO Validated] PFKP Rabbit pAb (A7916)
ABclonal:Immunofluorescence - [KO Validated] PFKP Rabbit pAb (A7916)

Overview

Product name[KO Validated] PFKP Rabbit pAb
Catalog No.A7916
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human PFKP
SequenceEmail for sequence
Gene ID5214
Swiss protQ01813
SynonymsPFKP; ATP-PFK; PFK-C; PFK-P; PFKF
Calculated MW85kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF-7, SKOV3, HeLa, NCI-H460, Mouse brain, Rat heart
Cellular locationCytoplasm
ABclonal:Western blot - [KO Validated] PFKP Rabbit pAb (A7916) }

Western blot - [KO Validated] PFKP Rabbit pAb (A7916)

Western blot analysis of extracts of various cell lines, using PFKP antibody (A7916) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] PFKP Rabbit pAb (A7916) }

Western blot - [KO Validated] PFKP Rabbit pAb (A7916)

Western blot analysis of extracts from normal (control) and PFKP knockout (KO) HeLa cells, using PFKP antibody (A7916) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] PFKP Rabbit pAb (A7916) }

Immunohistochemistry - [KO Validated] PFKP Rabbit pAb (A7916)

Immunohistochemistry of paraffin-embedded rat brain using PFKP antibody (A7916) (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] PFKP Rabbit pAb (A7916) }

Immunohistochemistry - [KO Validated] PFKP Rabbit pAb (A7916)

Immunohistochemistry of paraffin-embedded mouse brain using PFKP antibody (A7916) (40x lens).
ABclonal:Immunofluorescence - [KO Validated] PFKP Rabbit pAb (A7916) }

Immunofluorescence - [KO Validated] PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of U-2 OS cells using PFKP antibody (A7916) at dilution of 1:100. Blue: DAPI for nuclear staining.

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