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[KO Validated] PD-L1/CD274 Rabbit mAb (A19135)

Datasheet

ABclonal:Western blot - [KO Validated] PD-L1/CD274 Rabbit mAb (A19135)

Western blot analysis of extracts from normal (control) and PD-L1/CD274 knockout (KO) A-549 cells, using PD-L1/CD274 antibody (A19135) at 1:1000 dilution.Both A-549 cells and PD-L1/CD274 knockout (KO) A-549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] PD-L1/CD274 Rabbit mAb (A19135)

Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PD-L1/CD274 Rabbit mAb (A19135) at dilution of 1:100 (40x lens).

Overview

Product name[KO Validated] PD-L1/CD274 Rabbit mAb
Catalog No.A19135
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide of human PD-L1/CD274.
SequenceEmail for sequence
Gene ID29126
Swiss protQ9NZQ7
SynonymsB7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; CD274
Calculated MW20kDa/33kDa
Observed MW43-50kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549
Cellular locationCell membrane, Endomembrane system, Single-pass type I membrane protein
ABclonal:Western blot - [KO Validated] PD-L1/CD274 Rabbit mAb (A19135) }

Western blot - [KO Validated] PD-L1/CD274 Rabbit mAb (A19135)

Western blot analysis of extracts from normal (control) and PD-L1/CD274 knockout (KO) A-549 cells, using PD-L1/CD274 antibody (A19135) at 1:1000 dilution.Both A-549 cells and PD-L1/CD274 knockout (KO) A-549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] PD-L1/CD274 Rabbit mAb (A19135) }

Immunohistochemistry - [KO Validated] PD-L1/CD274 Rabbit mAb (A19135)

Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PD-L1/CD274 Rabbit mAb (A19135) at dilution of 1:100 (40x lens).

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