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[KO Validated] PDCD4 Rabbit pAb (A2570)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] PDCD4 Rabbit pAb (A2570)
ABclonal:Western blot - [KO Validated] PDCD4 Rabbit pAb (A2570)
ABclonal:Immunohistochemistry - [KO Validated] PDCD4 Rabbit pAb (A2570)
ABclonal:Immunohistochemistry - [KO Validated] PDCD4 Rabbit pAb (A2570)

Overview

Product name[KO Validated] PDCD4 Rabbit pAb
Catalog No.A2570
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
SequenceMDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQL
Gene ID27250
Swiss protQ53EL6
SynonymsPDCD4; H731
Calculated MW50kDa/51kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMCF-7, HeLa, HepG2, Mouse thymus
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Rattus norvegicus, Sus scrofa)

ABclonal:Western blot - [KO Validated] PDCD4 Rabbit pAb (A2570) }

Western blot - [KO Validated] PDCD4 Rabbit pAb (A2570)

Western blot analysis of extracts of various cell lines, using PDCD4 antibody (A2570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] PDCD4 Rabbit pAb (A2570) }

Western blot - [KO Validated] PDCD4 Rabbit pAb (A2570)

Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (A2570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] PDCD4 Rabbit pAb (A2570) }

Immunohistochemistry - [KO Validated] PDCD4 Rabbit pAb (A2570)

Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] PDCD4 Rabbit pAb (A2570) at dilution of 1:25 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] PDCD4 Rabbit pAb (A2570) }

Immunohistochemistry - [KO Validated] PDCD4 Rabbit pAb (A2570)

Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PDCD4 Rabbit pAb (A2570) at dilution of 1:25 (40x lens).

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