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[KO Validated] PDCD10 Rabbit pAb (A15400)

Datasheet

ABclonal:Western blot - [KO Validated] PDCD10 Rabbit pAb (A15400)
ABclonal:Western blot - [KO Validated] PDCD10 Rabbit pAb (A15400)

Overview

Product name[KO Validated] PDCD10 Rabbit pAb
Catalog No.A15400
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PDCD10 (NP_009148.2).
SequenceMRMTMEEMKNEAETTSMVSMPLYAVMYPVFNELERVNLSAAQTLRAAFIKAEKENPGLTQDIIMKILEKKSVEVNFTESLLRMAADDVEEYMIERPEPEFQDLNEKARALKQILSKIPDEINDRVRFLQTIKDIASAIKELLDTVNNVFKKYQYQNRRALEHQKKEFVKYSKSFSDTLKTYFKDGKAINVFVSANRLIHQTNLILQTFKTVA
Gene ID11235
Swiss protQ9BUL8
SynonymsPDCD10; CCM3; TFAR15
Calculated MW24kDa
Observed MW26KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesA-431, K-562, U-251MG, SKOV3, Mouse brain, Mouse kidney, Rat brain
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein
    ABclonal:Western blot - [KO Validated] PDCD10 Rabbit pAb (A15400) }

    Western blot - [KO Validated] PDCD10 Rabbit pAb (A15400)

    Western blot analysis of extracts from normal (control) and PDCD10 knockout (KO) HeLa cells, using PDCD10 antibody (A15400) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Western blot - [KO Validated] PDCD10 Rabbit pAb (A15400) }

    Western blot - [KO Validated] PDCD10 Rabbit pAb (A15400)

    Western blot analysis of extracts of various cell lines, using PDCD10 antibody (A15400) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

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