Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] PARK7 Rabbit pAb (A0987)

Datasheet

ABclonal:Western blot - [KO Validated] PARK7 Rabbit pAb (A0987)
ABclonal:Western blot - [KO Validated] PARK7 Rabbit pAb (A0987)
ABclonal:Immunofluorescence - [KO Validated] PARK7 Rabbit pAb (A0987)

Overview

Product name[KO Validated] PARK7 Rabbit pAb
Catalog No.A0987
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).
SequenceMASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLKD
Gene ID11315
Swiss protQ99497
SynonymsDJ-1; DJ1; GATD2; HEL-S-67p; PARK7
Calculated MW19kDa
Observed MW20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, Jurkat
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Membrane raft, Mitochondrion, Nucleus
ABclonal:Western blot - [KO Validated] PARK7 Rabbit pAb (A0987) }

Western blot - [KO Validated] PARK7 Rabbit pAb (A0987)

Western blot analysis of extracts of various cell lines, using PARK7 antibody (A0987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] PARK7 Rabbit pAb (A0987) }

Western blot - [KO Validated] PARK7 Rabbit pAb (A0987)

Western blot analysis of extracts from normal (control) and PARK7 knockout (KO) 293T cells, using PARK7 antibody (A0987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - [KO Validated] PARK7 Rabbit pAb (A0987) }

Immunofluorescence - [KO Validated] PARK7 Rabbit pAb (A0987)

Immunofluorescence analysis of HeLa cells using PARK7 antibody (A0987). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A0987 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order