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[KO Validated] P4HB Rabbit pAb (A0692)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Western blot - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Immunofluorescence - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Immunofluorescence - [KO Validated] P4HB Rabbit pAb (A0692)
ABclonal:Immunoprecipitation - [KO Validated] P4HB Rabbit pAb (A0692)

Overview

Product name[KO Validated] P4HB Rabbit pAb
Catalog No.A0692
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2).
SequencePESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL
Gene ID5034
Swiss protP07237
SynonymsP4HB; CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB
Calculated MW57kDa
Observed MW57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesTHP-1, SW620, CEM, NIH/3T3, RAW264.7, Mouse liver, Mouse kidney
Cellular locationCell membrane, Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Peripheral membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] P4HB Rabbit pAb (A0692) }

Western blot - [KO Validated] P4HB Rabbit pAb (A0692)

Western blot analysis of extracts of various cell lines, using P4HB antibody (A0692) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] P4HB Rabbit pAb (A0692) }

Western blot - [KO Validated] P4HB Rabbit pAb (A0692)

Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibody (A0692) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692) }

Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692)

Immunohistochemistry of paraffin-embedded human lung cancer using P4HB antibody (A0692) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692) }

Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692)

Immunohistochemistry of paraffin-embedded mouse brain using P4HB antibody (A0692) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692) }

Immunohistochemistry - [KO Validated] P4HB Rabbit pAb (A0692)

Immunohistochemistry of paraffin-embedded rat kidney using P4HB antibody (A0692) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] P4HB Rabbit pAb (A0692) }

Immunofluorescence - [KO Validated] P4HB Rabbit pAb (A0692)

Immunofluorescence analysis of HeLa cells using P4HB antibody (A0692). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] P4HB Rabbit pAb (A0692) }

Immunofluorescence - [KO Validated] P4HB Rabbit pAb (A0692)

Confocal immunofluorescence analysis of U-2OS cells using P4HB antibody (A0692) at dilution of 1:400. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] P4HB Rabbit pAb (A0692) }

Immunoprecipitation - [KO Validated] P4HB Rabbit pAb (A0692)

Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody (A0692). Western blot was performed from the immunoprecipitate using P4HB antibody (A0692) at a dilition of 1:500.

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