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[KO Validated] OXCT1 Rabbit pAb (A8139)

Datasheet

ABclonal:Western blot - [KO Validated] OXCT1 Polyclonal Antibody (A8139)
ABclonal:Western blot - [KO Validated] OXCT1 Polyclonal Antibody (A8139)
ABclonal:Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (A8139)
ABclonal:Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (A8139)

Overview

Product name[KO Validated] OXCT1 Rabbit pAb
Catalog No.A8139
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).
SequenceIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN
Gene ID5019
Swiss protP55809
SynonymsOXCT1; OXCT; SCOT
Calculated MW13kDa/56kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesBT-474, HeLa, Jurkat, 293T, Mouse kidney, Mouse heart, Rat kidney
Cellular locationMitochondrion matrix
ABclonal:Western blot - [KO Validated] OXCT1 Polyclonal Antibody (A8139) }

Western blot - [KO Validated] OXCT1 Polyclonal Antibody (A8139)

Western blot analysis of extracts of various cell lines, using OXCT1 antibody (A8139) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] OXCT1 Polyclonal Antibody (A8139) }

Western blot - [KO Validated] OXCT1 Polyclonal Antibody (A8139)

Western blot analysis of extracts from normal (control) and OXCT1 knockout (KO) HeLa cells, using OXCT1 antibody (A8139) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (A8139) }

Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (A8139)

Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody (A8139) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (A8139) }

Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (A8139)

Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody (A8139) at dilution of 1:100 (40x lens).

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