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[KO Validated] NUDT1 Rabbit pAb (A13330)

Datasheet

ABclonal:Western blot - [KO Validated] NUDT1 Polyclonal Antibody (A13330)
ABclonal:Western blot - [KO Validated] NUDT1 Polyclonal Antibody (A13330)
ABclonal:Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (A13330)
ABclonal:Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (A13330)

Overview

Product name[KO Validated] NUDT1 Rabbit pAb
Catalog No.A13330
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1).
SequenceMSGISPQQMGEPEGSWSGKNPGTMGASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPVESDEMRPCWFQLDQIPFKDMWPDDSYWFPLLLQKKKFHGYFKFQGQDTILDYTLREVDTV
Gene ID4521
Swiss protP36639
SynonymsNUDT1; MTH1
Calculated MW17kDa/19kDa/20kDa/22kDa
Observed MW18kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesSW480, K562, 22RV1, THP-1, HepG2, SKOV3, 293T
Cellular locationCytoplasm, Mitochondrion matrix, Nucleus
ABclonal:Western blot - [KO Validated] NUDT1 Polyclonal Antibody (A13330) }

Western blot - [KO Validated] NUDT1 Polyclonal Antibody (A13330)

Western blot analysis of extracts of various cell lines, using NUDT1 antibody (A13330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] NUDT1 Polyclonal Antibody (A13330) }

Western blot - [KO Validated] NUDT1 Polyclonal Antibody (A13330)

Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 antibody (A13330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (A13330) }

Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (A13330)

Immunohistochemistry of paraffin-embedded human gastric cancer using NUDT1 antibody (A13330) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (A13330) }

Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (A13330)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug NUDT1 antibody (A13330). Western blot was performed from the immunoprecipitate using NUDT1 antibody (A13330) at a dilition of 1:1000.

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