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[KO Validated] NOX4 Rabbit pAb (A11274)

Publications (8) Datasheet

ABclonal:Western blot - [KO Validated] NOX4 Rabbit pAb (A11274)

Western blot analysis of extracts of 293T cells, using NOX4 antibody (A11274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] NOX4 Rabbit pAb (A11274)

Western blot analysis of extracts from normal (control) and NOX4 Rabbit pAb knockout (KO) HeLa cells, using NOX4 Rabbit pAb antibody (A11274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KO Validated] NOX4 Rabbit pAb (A11274)

Immunohistochemistry of paraffin-embedded rat lung using NOX4 Rabbit pAb (A11274) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] NOX4 Rabbit pAb (A11274)

Immunohistochemistry of paraffin-embedded rat kidney using NOX4 Rabbit pAb (A11274) at dilution of 1:100 (40x lens).

Overview

Product name[KO Validated] NOX4 Rabbit pAb
Catalog No.A11274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 328-578 of human NOX4 (NP_058627.1).
SequenceKENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS
Gene ID50507
Swiss protQ9NPH5
SynonymsNOX4; KOX; KOX-1; RENOX
Calculated MW6kDa/25-31kDa/58-66kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T
Cellular locationCell junction, Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, Nucleus, focal adhesion, nucleolus
Customer validation

WB(Homo sapiens,Rattus norvegicus, Mus musculus)

ABclonal:Western blot - [KO Validated] NOX4 Rabbit pAb (A11274) }

Western blot - [KO Validated] NOX4 Rabbit pAb (A11274)

Western blot analysis of extracts of 293T cells, using NOX4 antibody (A11274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] NOX4 Rabbit pAb (A11274) }

Western blot - [KO Validated] NOX4 Rabbit pAb (A11274)

Western blot analysis of extracts from normal (control) and NOX4 Rabbit pAb knockout (KO) HeLa cells, using NOX4 Rabbit pAb antibody (A11274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KO Validated] NOX4 Rabbit pAb (A11274) }

Immunohistochemistry - [KO Validated] NOX4 Rabbit pAb (A11274)

Immunohistochemistry of paraffin-embedded rat lung using NOX4 Rabbit pAb (A11274) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NOX4 Rabbit pAb (A11274) }

Immunohistochemistry - [KO Validated] NOX4 Rabbit pAb (A11274)

Immunohistochemistry of paraffin-embedded rat kidney using NOX4 Rabbit pAb (A11274) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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