Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

[KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Tested applications:WB IHC ICC IF/ICC IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat, Monkey

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded Human colon using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Human tonsil using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilution of 1:1000.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Chromatin immunoprecipitation analysis of extracts of HT-1080 treated by TNF-α, using [KO Validated] NF-kB p65/RelA Rabbit mAb antibody (A19653) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review

Overview

Product name	[KO Validated] NF-kB p65/RelA Rabbit mAb
Catalog No.	A19653
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51086

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-κB p65 (Q04206).
Sequence	LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
Gene ID	5970
Swiss prot	Q04206
Synonyms	NFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW	65kDa
Observed MW	65KDa

Applications

Reactivity	Human, Mouse, Rat, Monkey
Tested applications	WB IHC ICC IF/ICC IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	MCF7, 293T, Mouse spleen, Mouse liver, HeLa, NIH/3T3, Mouse lung, PC-12
Cellular location	Cytoplasm, Nucleus
Customer validation	(RPMI-8226、PC-3、HepG2、Hela、U2-OS、Jurkat、MOLT-4) WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Homo sapiens) IHC(Rattus norvegicus, A. baumannii) WB(Homo sapiens) IF(Rattus norvegicus, Mus musculus ) WB，IF(Rattus norvegicus) IP(Mus musculus )

Research Area

[KO Validated] NF-kB p65/RelA Rabbit mAb images

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of U2OS using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

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References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Inflammation

Application:

IF:

4.09

Species:

Mus musculus

PMID:

34341910

Title:

LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

J Nat Med

Application:

IF:

2.343

Species:

Mus musculus

PMID:

32761488

Title:

Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF-κB signaling pathway activation: an in vivo and in vitro study

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Life Sci

Application:

IF:

5.037

Species:

Homo sapiens

PMID:

32971102

Title:

PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

33708105

Title:

Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Cell Death Discov

Application:

IF:

5.241

Species:

Homo sapiens

PMID:

33597510

Title:

Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Mol Cell Biochem

Application:

IF:

3.396

Species:

Rattus norvegicus

PMID:

33608832

Title:

Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium-glucose cotransporter 1

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens,Mus musculus

PMID:

34108444

Title:

SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Mol Med Rep

Application:

IF:

2.952

Species:

Mus musculus

PMID:

34132358

Title:

MicroRNA?16?5p/BIMP1/NF?κB axis regulates autophagy to exert a tumor?suppressive effect on bladder cancer

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Int J Mol Med

Application:

IF:

4.101

Species:

Mus musculus

PMID:

34751408

Title:

BAY61?3606 attenuates neuroinflammation and neurofunctional damage by inhibiting microglial Mincle/Syk signaling response after traumatic brain injury

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

J Neuroimmune Pharmacol

Application:

IF:

4.147

Species:

Mus musculus

PMID:

34727296

Title:

STING/NF-κB/IL-6-Mediated Inflammation in Microglia Contributes to Spared Nerve Injury (SNI)-Induced Pain Initiation

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

6.92

Species:

Rattus norvegicus

PMID:

35002280

Title:

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Life sciences

Application:

IF:

5.03

Species:

Mus musculus

PMID:

33508297

Title:

Inhibition of SGLT1 protects against glycemic variability-induced cardiac damage and pyroptosis of cardiomyocytes in diabetic mice

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Biological trace element research

Application:

IHC

IF:

3.73

Species:

Rattus norvegicus

PMID:

27283837

Title:

Quercetin and Vitamin C Mitigate Cobalt Chloride-Induced Hypertension through Reduction in Oxidative Stress and Nuclear Factor Kappa Beta (NF-Kb) Expression in Experimental Rat Model

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF, WB

IF:

7.56

Species:

Homo sapiens

PMID:

35386692

Title:

AQP2 Promotes Astrocyte Activation by Modulating the TLR4/NFκB-p65 Pathway Following Intracerebral Hemorrhage

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

4.35

Species:

Rattus norvegicus

PMID:

35327312

Title:

The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Mus musculus

PMID:

35273604

Title:

Amelioration of Lupus Serum-Induced Skin Inflammation in CD64-Deficient Mice

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.587

Species:

Mus musculus

PMID:

35093117

Title:

AMPK-autophagy-mediated inhibition of microRNA-30a-5p alleviates morphine tolerance via SOCS3-dependent neuroinflammation suppression

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

4.63

Species:

Rattus norvegicus

PMID:

35494314

Title:

Inhibition of miRNA-1-Mediated Inflammation and Autophagy by Astragaloside IV Improves Lipopolysaccharide-Induced Cardiac Dysfunction in Rats

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Bioorganic & medicinal chemistry

Application:

IF:

3.46

Species:

Mus musculus

PMID:

33223464

Title:

Synthesis of nature product kinsenoside analogues with anti-inflammatory activity

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IHC

IF:

5.21

Species:

A. baumannii

PMID:

35631375

Title:

Mechanistic Insights on the In Vitro Antibacterial Activity and In Vivo Hepatoprotective Effects of Salvinia auriculata Aubl against Methotrexate-Induced Liver Injury

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Experimental cell research

Application:

WB, IF

IF:

4.14

Species:

Rattus norvegicus

PMID:

28988741

Title:

Smad7 alleviates glomerular mesangial cell proliferation via the ROS-NF-κB pathway

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Journal of biochemical and molecular toxicology

Application:

WB，IF

IF:

3.56

Species:

Rattus norvegicus

PMID:

36184831

Title:

Emodin suppresses oxaliplatin‐induced neuropathic pain by inhibiting COX2/NF‐κB mediated spinal inflammation

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Mus musculus

PMID:

36235742

Title:

Protective Effect of Rhus chinensis Mill. Fruits on 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine-Induced Cholestasis in Mice via Ameliorating Oxidative Stress and Inflammation - PMC

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Life sciences

Application:

IHC

IF:

6.78

Species:

Rattus norvegicus

PMID:

36209830

Title:

Agmatine-mediated inhibition of NMDA receptor expression and amelioration of dyskinesia via activation of Nrf2 and suppression of HMGB1/RAGE/TLR4/MYD88/NF …

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

36228892

Title:

Efficacy of curculigoside in protecting against ischemic brain injury through regulation of oxidative stress and NF-κB and PI3K/Akt expression

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Homo sapiens

PMID:

35785707

Title:

Trichodermin inhibits the growth of oral cancer through apoptosis-induced mitochondrial dysfunction and HDAC-2-mediated signaling

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

35260874

Title:

Lycopene Ameliorates Atrazine-Induced Pyroptosis in Spleen via Suppressing the Ox-mtDNA/Nlrp3 Inflammasome Pathway

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Acta tropica

Application:

IP, IF

IF:

3.22

Species:

Mus musculus

PMID:

35963313

Title:

Toxoplasma gondii rhoptry protein (TgROP18) enhances the expression of pro-inflammatory factor in LPS/IFN-γ-induced murine BV2 microglia cells via NF-κB signal …

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36232498

Title:

The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-κB Signalling - PMC

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Drug design, development and therapy

Application:

IHC

IF:

4.31

Species:

Rattus norvegicus

PMID:

36105322

Title:

Alpha-Chymotrypsin Protects Against Acute Lung, Kidney, and Liver Injuries and Increases Survival in CLP-Induced Sepsis in Rats Through Inhibition of TLR4/NF-κB Pathway - PMC

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IHC

IF:

5.21

Species:

Rattus norvegicus

PMID:

35893742

Title:

Activation of SIRT-1 Pathway by Nanoceria Sheds Light on Its Ameliorative Effect on Doxorubicin-Induced Cognitive Impairment (Chemobrain): Restraining Its Neuroinflammation, Synaptic Dysplasticity and Apoptosis - PMC