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[KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat, Monkey

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded rat spleen using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human lung cancer using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilition of 1:1000.

Overview

Product name	[KO Validated] NF-kB p65/RelA Rabbit mAb
Catalog No.	A19653
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Immunogen information

Immunogen	A synthesized peptide derived from human NF-κB p65.
Sequence	Email for sequence
Gene ID	5970
Swiss prot	Q04206
Synonyms	NFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW	65kDa
Observed MW	65KDa

Applications

Reactivity	Human, Mouse, Rat, Monkey
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	MCF7, 293T, Mouse spleen, Mouse liver, HeLa
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Homo sapiens)

Research Area

[KO Validated] NF-kB p65/RelA Rabbit mAb images

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product Protocols

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References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Inflammation

Application:

IF:

4.09

Species:

Mus musculus

PMID:

34341910

Title:

LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

J Nat Med

Application:

IF:

2.343

Species:

Mus musculus

PMID:

32761488

Title:

Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF-κB signaling pathway activation: an in vivo and in vitro study

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Life Sci

Application:

IF:

5.037

Species:

Homo sapiens

PMID:

32971102

Title:

PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

33708105

Title:

Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Cell Death Discov

Application:

IF:

5.241

Species:

Homo sapiens

PMID:

33597510

Title:

Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Mol Cell Biochem

Application:

IF:

3.396

Species:

Rattus norvegicus

PMID:

33608832

Title:

Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium-glucose cotransporter 1

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens,Mus musculus

PMID:

34108444

Title:

SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Mol Med Rep

Application:

IF:

2.952

Species:

Mus musculus

PMID:

34132358

Title:

MicroRNA?16?5p/BIMP1/NF?κB axis regulates autophagy to exert a tumor?suppressive effect on bladder cancer

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Int J Mol Med

Application:

IF:

4.101

Species:

Mus musculus

PMID:

34751408

Title:

BAY61?3606 attenuates neuroinflammation and neurofunctional damage by inhibiting microglial Mincle/Syk signaling response after traumatic brain injury

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

J Neuroimmune Pharmacol

Application:

IF:

4.147

Species:

Mus musculus

PMID:

34727296