Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

[KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Review (1)Publications (31) Datasheet

Tested applications:WBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded Human colon using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded Human tonsil using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of U2OS using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilution of 1:1000.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Chromatin immunoprecipitation analysis of extracts of HT-1080 treated by TNF-α, using [KO Validated] NF-kB p65/RelA Rabbit mAb antibody (A19653) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for [KO Validated] NF-kB p65/RelA Rabbit mAb(A19653)

Overview

Product name[KO Validated] NF-kB p65/RelA Rabbit mAb
Catalog No.A19653
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51086
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-κB p65 (Q04206).
SequenceLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW65kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesMCF7, 293T, Mouse spleen, Mouse liver, HeLa, NIH/3T3, Mouse lung, PC-12
Cellular locationCytoplasm, Nucleus
Customer validation

(RPMI-8226、PC-3、HepG2、Hela、U2-OS、Jurkat、MOLT-4)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Homo sapiens)

IHC(Rattus norvegicus, A. baumannii)

WB(Homo sapiens)

IF(Rattus norvegicus, Mus musculus )

WB,IF(Rattus norvegicus)

IP(Mus musculus )

ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded Human colon using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunohistochemistry of paraffin-embedded Human tonsil using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of NIH/3T3 using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunofluorescence analysis of U2OS using [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilution of 1:1000.
ABclonal:Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)}

Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A19653)

Chromatin immunoprecipitation analysis of extracts of HT-1080 treated by TNF-α, using [KO Validated] NF-kB p65/RelA Rabbit mAb antibody (A19653) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Primary Antibodies (19)

Secondary Antibodies (18)

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