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[KO Validated] NFKB1 Polyclonal Antibody (A6667)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] NFKB1 Polyclonal Antibody (A6667)
ABclonal:Western blot - [KO Validated] NFKB1 Polyclonal Antibody (A6667)
ABclonal:Immunohistochemistry - [KO Validated] NFKB1 Polyclonal Antibody (A6667)
ABclonal:Immunofluorescence - [KO Validated] NFKB1 Polyclonal Antibody (A6667)
Review
ABclonal: review for [KO Validated] NFKB1 Polyclonal Antibody(A6667)
Review
ABclonal: review for [KO Validated] NFKB1 Polyclonal Antibody(A6667)

Overview

Product name[KO Validated] NFKB1 Polyclonal Antibody
Catalog No.A6667
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).
SequenceAAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQGDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPL
Gene ID4790
Swiss protP19838
SynonymsNFKB1; CVID12; EBP-1; KBF1; NF-kB1; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; NFkappaB; p105; p50; nuclear factor kappa B subunit 1
Calculated MW85kDa/105kDa
Observed MW120kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, Mouse thymus
Cellular locationCytoplasm, Nucleus
Customer validation

(, )

WB(Mouse, Human)

IF(Mouse)

ABclonal:Western blot - [KO Validated] NFKB1 Polyclonal Antibody (A6667) }

Western blot - [KO Validated] NFKB1 Polyclonal Antibody (A6667)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody (A6667) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] NFKB1 Polyclonal Antibody (A6667) }

Western blot - [KO Validated] NFKB1 Polyclonal Antibody (A6667)

Western blot analysis of extracts from normal (control) and NFKB1 knockout (KO) HeLa cells, using NFKB1 antibody (A6667) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] NFKB1 Polyclonal Antibody (A6667) }

Immunohistochemistry - [KO Validated] NFKB1 Polyclonal Antibody (A6667)

Immunohistochemistry of paraffin-embedded mouse spleen using NFKB1 antibody (A6667) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] NFKB1 Polyclonal Antibody (A6667) }

Immunofluorescence - [KO Validated] NFKB1 Polyclonal Antibody (A6667)

Immunofluorescence analysis of A549 cells using NFKB1 antibody (A6667).

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