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[KO Validated] NF2 Rabbit pAb (A0739)

Datasheet

ABclonal:Western blot - [KO Validated] NF2 Rabbit pAb (A0739)
ABclonal:Western blot - [KO Validated] NF2 Rabbit pAb (A0739)
ABclonal:Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739)
ABclonal:Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739)
ABclonal:Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739)
ABclonal:Immunoprecipitation - [KO Validated] NF2 Rabbit pAb (A0739)

Overview

Product name[KO Validated] NF2 Rabbit pAb
Catalog No.A0739
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1).
SequenceTKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNT
Gene ID4771
Swiss protP35240
SynonymsNF2; ACN; BANF; SCH; merlin
Calculated MW19-30kDa/59-72kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesA549, A431, HeLa, SKOV3, Mouse kidney, Mouse heart, Rat brain
Cellular locationCell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane
ABclonal:Western blot - [KO Validated] NF2 Rabbit pAb (A0739) }

Western blot - [KO Validated] NF2 Rabbit pAb (A0739)

Western blot analysis of extracts of various cell lines, using NF2 antibody (A0739) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] NF2 Rabbit pAb (A0739) }

Western blot - [KO Validated] NF2 Rabbit pAb (A0739)

Western blot analysis of extracts from normal (control) and NF2 knockout (KO) HeLa cells, using NF2 antibody (A0739) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 6s.
ABclonal:Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739) }

Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739)

Immunofluorescence analysis of C6 cells using NF2 antibody (A0739) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739) }

Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739)

Immunofluorescence analysis of L929 cells using NF2 antibody (A0739) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739) }

Immunofluorescence - [KO Validated] NF2 Rabbit pAb (A0739)

Immunofluorescence analysis of U2OS cells using NF2 antibody (A0739) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] NF2 Rabbit pAb (A0739) }

Immunoprecipitation - [KO Validated] NF2 Rabbit pAb (A0739)

Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2 antibody (A0739). Western blot was performed from the immunoprecipitate using NF2 antibody (A0739) at a dilition of 1:1000.

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