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[KO Validated] NDUFS3 Rabbit pAb (A8013)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)
ABclonal:Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)
ABclonal:Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)
ABclonal:Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)

Overview

Product name[KO Validated] NDUFS3 Rabbit pAb
Catalog No.A8013
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
SequenceESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK
Gene ID4722
Swiss protO75489
SynonymsNDUFS3; CI-30
Calculated MW14kDa/30kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMCF-7, SW620, HepG2, Mouse kidney, Mouse liver, Mouse heart, Rat liver, Rat heart
Cellular locationMitochondrion inner membrane
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (A8013) }

Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)

Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (A8013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (A8013) }

Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)

Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (A8013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (A8013) }

Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)

Immunohistochemistry of paraffin-embedded human liver damage using NDUFS3 antibody (A8013) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (A8013) }

Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (A8013)

Immunohistochemistry of paraffin-embedded rat brain using NDUFS3 antibody (A8013) at dilution of 1:100 (40x lens).

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