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[KO Validated] NDUFS2 Rabbit pAb (A12858)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (A12858)
ABclonal:Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (A12858)
ABclonal:Immunofluorescence - [KO Validated] NDUFS2 Polyclonal Antibody (A12858)

Overview

Product name[KO Validated] NDUFS2 Rabbit pAb
Catalog No.A12858
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1).
SequenceFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLMDDIYQFSKNFSLRLDELEELLTNNRIWRNRTIDIGVVTAEEALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPVGSRGDCYDRYLCRVEEMRQSLRIIAQCLNKMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR
Gene ID4720
Swiss protO75306
SynonymsNDUFS2; CI-49
Calculated MW51kDa/52kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesLO2, HeLa, 293T, Mouse brain, Mouse kidney, Mouse spleen, Rat brain
Cellular locationMatrix side, Mitochondrion inner membrane, Peripheral membrane protein
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (A12858) }

Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (A12858)

Western blot analysis of extracts of various cell lines, using NDUFS2 antibody (A12858) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (A12858) }

Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (A12858)

Western blot analysis of extracts from normal (control) and NDUFS2 knockout (KO) 293T cells, using NDUFS2 antibody (A12858) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - [KO Validated] NDUFS2 Polyclonal Antibody (A12858) }

Immunofluorescence - [KO Validated] NDUFS2 Polyclonal Antibody (A12858)

Immunofluorescence analysis of L929 cells using NDUFS2 antibody (A12858) at dilution of 1:100. Blue: DAPI for nuclear staining.

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