Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] NDUFC2 Rabbit pAb (A15073)

Datasheet

ABclonal:Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (A15073)
ABclonal:Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (A15073)

Overview

Product name[KO Validated] NDUFC2 Rabbit pAb
Catalog No.A15073
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
SequenceMIARRNPEPLRFLPDEARSLPPPKLTDPRLLYIGFLGYCSGLIDNLIRRRPIATAGLHRQLLYITAFFFAGYYLVKREDYLYAVRDREMFGYMKLHPEDF
Gene ID4718
Swiss protO95298
SynonymsNDUFC2; B14.5b; CI-B14.5b; HLC-1; NADHDH2
Calculated MW10kDa/11kDa/13kDa/14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, OVCAR3, HeLa, A-549, mouse stomach, mouse brain, mouse heart, rat kidney
Cellular locationMatrix side, Mitochondrion inner membrane, Single-pass membrane protein
ABclonal:Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (A15073) }

Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (A15073)

Western blot analysis of extracts of various cell lines, using NDUFC2 antibody (A15073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (A15073) }

Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (A15073)

Western blot analysis of extracts from normal (control) and NDUFC2 knockout (KO) HeLa cells, using NDUFC2 antibody (A15073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Inquire About This Product

Submit your question about A15073 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order