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[KO Validated] NDUFB4 Rabbit pAb (A13820)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] NDUFB4 Rabbit pAb (A13820)
ABclonal:Western blot - [KO Validated] NDUFB4 Rabbit pAb (A13820)
ABclonal:Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820)
ABclonal:Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820)
ABclonal:Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820)
ABclonal:Immunofluorescence - [KO Validated] NDUFB4 Rabbit pAb (A13820)
ABclonal:Immunofluorescence - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Overview

Product name[KO Validated] NDUFB4 Rabbit pAb
Catalog No.A13820
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
SequenceMSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMG
Gene ID4710
Swiss protO95168
SynonymsNDUFB4; B15; CI-B15
Calculated MW14kDa/15kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, HeLa, 293T, Mouse heart, Mouse kidney, Mouse liver
Cellular locationMatrix side, Mitochondrion inner membrane, Single-pass membrane protein
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Western blot - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Western blot analysis of extracts of various cell lines, using NDUFB4 antibody (A13820) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Western blot - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using NDUFB4 antibody (A13820) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Immunohistochemistry of paraffin-embedded human liver using [KO Validated] NDUFB4 Rabbit pAb (A13820) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] NDUFB4 Rabbit pAb (A13820) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Immunohistochemistry - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] NDUFB4 Rabbit pAb (A13820) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Immunofluorescence - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Immunofluorescence analysis of L929 cells using NDUFB4 Polyclonal Antibody (A13820) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] NDUFB4 Rabbit pAb (A13820) }

Immunofluorescence - [KO Validated] NDUFB4 Rabbit pAb (A13820)

Immunofluorescence analysis of U-2 OS cells using NDUFB4 Polyclonal Antibody (A13820) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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