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[KO Validated] NDUFA7 Rabbit pAb (A8441)

Datasheet

ABclonal:Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (A8441)
ABclonal:Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (A8441)
ABclonal:Immunohistochemistry - [KO Validated] NDUFA7 Polyclonal Antibody (A8441)

Overview

Product name[KO Validated] NDUFA7 Rabbit pAb
Catalog No.A8441
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
SequenceMASATRLIQRLRNWASGHDLQGKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRRESVPPSIIMSSQKALVSGKPAESSAVAATEKKAVTPAPPIKRWELSSDQPYL
Gene ID4701
Swiss protO95182
SynonymsNDUFA7; B14.5a; CI-B14.5a
Calculated MW12kDa
Observed MW13kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549, HepG2, MCF-7, Mouse kidney, Mouse brain, Mouse liver, Mouse heart, Rat liver
Cellular locationMatrix side, Mitochondrion inner membrane, Peripheral membrane protein
ABclonal:Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (A8441) }

Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (A8441)

Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (A8441) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (A8441) }

Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (A8441)

Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (A8441) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] NDUFA7 Polyclonal Antibody (A8441) }

Immunohistochemistry - [KO Validated] NDUFA7 Polyclonal Antibody (A8441)

Immunohistochemistry of paraffin-embedded human liver damage using NDUFA7 antibody (A8441) at dilution of 1:100 (40x lens).

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