Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] NDUFA5 Rabbit pAb (A14751)

Datasheet

ABclonal:Western blot - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)
ABclonal:Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)
ABclonal:Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)
ABclonal:Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)
ABclonal:Immunofluorescence - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)

Overview

Product name[KO Validated] NDUFA5 Rabbit pAb
Catalog No.A14751
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
SequenceMAGVLKKTTGLVGLAVCNTPHERLRILYTKILDVLEEIPKNAAYRKYTEQITNEKLAMVKAEPDVKKLEDQLQGGQLEEVILQAEHELNLARKMREWKLWEPLVEEPPADQWKWPI
Gene ID4698
Swiss protQ16718
SynonymsNDUFA5; B13; CI-13KD-B; CI-13kB; NUFM; UQOR13
Calculated MW13kDa
Observed MW13kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationMatrix side, Mitochondrion inner membrane, Peripheral membrane protein
ABclonal:Western blot - [KO Validated] NDUFA5 Polyclonal Antibody (A14751) }

Western blot - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)

Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody (A14751) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751) }

Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)

Immunohistochemistry of paraffin-embedded human tonsil using NDUFA5 antibody (A14751) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751) }

Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)

Immunohistochemistry of paraffin-embedded human stomach using NDUFA5 antibody (A14751) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751) }

Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)

Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFA5 antibody (A14751) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] NDUFA5 Polyclonal Antibody (A14751) }

Immunofluorescence - [KO Validated] NDUFA5 Polyclonal Antibody (A14751)

Immunofluorescence analysis of NIH-3T3 cells using NDUFA5 Polyclonal Antibody (A14751) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A14751 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order