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[KO Validated] N-Cadherin Rabbit pAb (A3045)

Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045)
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045)
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045)
ABclonal:Immunoprecipitation - [KO Validated] N-Cadherin Rabbit pAb (A3045)

Overview

Product name[KO Validated] N-Cadherin Rabbit pAb
Catalog No.A3045
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin (NP_001783.2).
SequenceDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAP
Gene ID1000
Swiss protP19022
SynonymsCD325; CDHN; CDw325; NCAD; N Cadherin; CDH2
Calculated MW97kDa/99kDa
Observed MW80kDa/135kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa, A-549, 293T, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat heart
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045) }

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A3045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045) }

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045)

Western blot analysis of extracts of HeLa cells, using N-Cadherin antibody (A3045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045) }

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A3045)

Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A3045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunoprecipitation - [KO Validated] N-Cadherin Rabbit pAb (A3045) }

Immunoprecipitation - [KO Validated] N-Cadherin Rabbit pAb (A3045)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug N-Cadherin antibody (A3045). Western blot was performed from the immunoprecipitate using N-Cadherin antibody (A3045) at a dilition of 1:1000.

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