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[KO Validated] NCOA4 Rabbit pAb (A5695)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] NCOA4 Rabbit pAb (A5695)
ABclonal:Western blot - [KO Validated] NCOA4 Rabbit pAb (A5695)
ABclonal:Immunohistochemistry - [KO Validated] NCOA4 Rabbit pAb (A5695)

Overview

Product name[KO Validated] NCOA4 Rabbit pAb
Catalog No.A5695
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 495-614 of human NCOA4 (NP_005428.1).
SequenceSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQM
Gene ID8031
Swiss protQ13772
SynonymsNCOA4; ARA70; ELE1; PTC3; RFG
Calculated MW32kDa/69kDa/71kDa/73kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA375, HeLa, MCF7, 293T, Mouse testis, Rat testis
Cellular location
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] NCOA4 Rabbit pAb (A5695) }

Western blot - [KO Validated] NCOA4 Rabbit pAb (A5695)

Western blot analysis of extracts of various cell lines, using NCOA4 antibody (A5695) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] NCOA4 Rabbit pAb (A5695) }

Western blot - [KO Validated] NCOA4 Rabbit pAb (A5695)

Western blot analysis of extracts from normal (control) and NCOA4 knockout (KO) HeLa cells, using NCOA4 antibody (A5695) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] NCOA4 Rabbit pAb (A5695) }

Immunohistochemistry - [KO Validated] NCOA4 Rabbit pAb (A5695)

Immunohistochemistry of paraffin-embedded human lung cancer using NCOA4 antibody (A5695) at dilution of 1:100 (40x lens).

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