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[KO Validated] NCOA2 Rabbit pAb (A10280)

Datasheet

ABclonal:Western blot - [KO Validated] NCOA2 Rabbit pAb (A10280)
ABclonal:Western blot - [KO Validated] NCOA2 Rabbit pAb (A10280)
ABclonal:Immunohistochemistry - [KO Validated] NCOA2 Rabbit pAb (A10280)
ABclonal:Immunohistochemistry - [KO Validated] NCOA2 Rabbit pAb (A10280)

Overview

Product name[KO Validated] NCOA2 Rabbit pAb
Catalog No.A10280
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1).
SequenceSSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQ
Gene ID10499
Swiss protQ15596
SynonymsNCOA2; GRIP1; KAT13C; NCoA-2; SRC2; TIF2; bHLHe75
Calculated MW159kDa
Observed MW200kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, BT-474
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] NCOA2 Rabbit pAb (A10280) }

Western blot - [KO Validated] NCOA2 Rabbit pAb (A10280)

Western blot analysis of extracts from normal (control) and NCOA2 knockout (KO) 293T cells, using NCOA2 antibody (A10280) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] NCOA2 Rabbit pAb (A10280) }

Western blot - [KO Validated] NCOA2 Rabbit pAb (A10280)

Western blot analysis of extracts of various cell lines, using NCOA2 antibody (A10280) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] NCOA2 Rabbit pAb (A10280) }

Immunohistochemistry - [KO Validated] NCOA2 Rabbit pAb (A10280)

Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] NCOA2 Rabbit pAb (A10280) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] NCOA2 Rabbit pAb (A10280) }

Immunohistochemistry - [KO Validated] NCOA2 Rabbit pAb (A10280)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] NCOA2 Rabbit pAb (A10280) at dilution of 1:100 (40x lens).

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