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[KO Validated] NBN Rabbit pAb (A0783)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] NBN Rabbit pAb (A0783)
ABclonal:Western blot - [KO Validated] NBN Rabbit pAb (A0783)
ABclonal:Immunohistochemistry - [KO Validated] NBN Rabbit pAb (A0783)
Review
ABclonal: review for [KO Validated] NBN Rabbit pAb(A0783)

Overview

Product name[KO Validated] NBN Rabbit pAb
Catalog No.A0783
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).
SequenceKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDSLWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLPHIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR
Gene ID4683
Swiss protO60934
SynonymsNBN; AT-V1; AT-V2; ATV; NBS; NBS1; P95; nibrin
Calculated MW84kDa
Observed MW95kDa/70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse testis, Rat testis
Cellular locationChromosome, Nucleus, PML body, telomere
Customer validation

(Mouse adipose-derived stem cells)

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] NBN Rabbit pAb (A0783) }

Western blot - [KO Validated] NBN Rabbit pAb (A0783)

Western blot analysis of extracts of various cell lines, using NBN antibody (A0783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] NBN Rabbit pAb (A0783) }

Western blot - [KO Validated] NBN Rabbit pAb (A0783)

Western blot analysis of extracts from normal (control) and NBN knockout (KO) 293T cells, using NBN antibody (A0783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] NBN Rabbit pAb (A0783) }

Immunohistochemistry - [KO Validated] NBN Rabbit pAb (A0783)

Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] NBN Rabbit pAb (A0783) at dilution of 1:50 (40x lens).

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