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[KO Validated] NBN Polyclonal Antibody (A0783)

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ABclonal:Western blot - [KO Validated] NBN Polyclonal Antibody (A0783)
ABclonal:Western blot - [KO Validated] NBN Polyclonal Antibody (A0783)
Review
ABclonal:Western blot (WB) review for [KO Validated] NBN Polyclonal Antibody(A0783)

Overview

Product name[KO Validated] NBN Polyclonal Antibody
Catalog No.A0783
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).
SequenceKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDSLWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLPHIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR
Gene ID4683
Swiss protO60934
SynonymsNBN; AT-V1; AT-V2; ATV; NBS; NBS1; P95; nibrin
Calculated MW84kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-87MG, HeLa, HepG2, mouse liver
Cellular locationChromosome, Nucleus, PML body, telomere
Customer validation

WB(Mouse adipose-derived stem cells, Human)

ABclonal:Western blot - [KO Validated] NBN Polyclonal Antibody (A0783) }

Western blot - [KO Validated] NBN Polyclonal Antibody (A0783)

Western blot analysis of extracts of various cell lines, using NBN antibody (A0783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] NBN Polyclonal Antibody (A0783) }

Western blot - [KO Validated] NBN Polyclonal Antibody (A0783)

Western blot analysis of extracts from normal (control) and NBN knockout (KO) HeLa cells, using NBN antibody (A0783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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