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[KO Validated] MLST8 Rabbit pAb (A1059)

Datasheet

ABclonal:Western blot - [KO Validated] MLST8 Polyclonal Antibody (A1059)
ABclonal:Western blot - [KO Validated] MLST8 Polyclonal Antibody (A1059)

Overview

Product name[KO Validated] MLST8 Rabbit pAb
Catalog No.A1059
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human MLST8 (NP_001186103.1).
SequenceMNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG
Gene ID64223
Swiss protQ9BVC4
SynonymsMLST8; GBL; GbetaL; LST8; POP3; WAT1
Calculated MW23kDa/28kDa/35kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, HeLa, K-562, 22Rv1, Mouse brain, Mouse kidney, Mouse thymus, Rat brain, Rat testis
Cellular locationCytoplasm
ABclonal:Western blot - [KO Validated] MLST8 Polyclonal Antibody (A1059) }

Western blot - [KO Validated] MLST8 Polyclonal Antibody (A1059)

Western blot analysis of extracts of various cell lines, using MLST8 antibody (A1059) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] MLST8 Polyclonal Antibody (A1059) }

Western blot - [KO Validated] MLST8 Polyclonal Antibody (A1059)

Western blot analysis of extracts from normal (control) and MLST8 knockout (KO) HeLa cells, using MLST8 antibody (A1059) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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