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[KO Validated] MLKL Rabbit pAb (A13451)

Datasheet

ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

Western blot analysis of extracts from normal (control) and MLKL knockout (KO) HeLa cells, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

Western blot analysis of extracts of various cell lines, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

Western blot analysis of extracts of Mouse liver, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

Western blot analysis of extracts of Rat testis, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] MLKL Rabbit pAb (A13451)

Immunohistochemistry of paraffin-embedded human breast cancer using MLKL antibody (A13451) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] MLKL Rabbit pAb (A13451)

Immunohistochemistry of paraffin-embedded human liver cancer using MLKL Rabbit pAb (A13451) at dilution of 1:200 (40x lens).

Overview

Product name[KO Validated] MLKL Rabbit pAb
Catalog No.A13451
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
ImmunogenRecombinant protein of mouse MLKL
SequenceEmail for sequence
Gene ID74568
Swiss protQ9D2Y4
SynonymsMLKL; hMLKL
Calculated MW53kDa/54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, NCI-H460, Mouse liver, Rat testis
Cellular locationCell membrane, Cytoplasm
    ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451) }

    Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts from normal (control) and MLKL knockout (KO) HeLa cells, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451) }

    Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts of various cell lines, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451) }

    Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts of Mouse liver, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - [KO Validated] MLKL Rabbit pAb (A13451) }

    Western blot - [KO Validated] MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts of Rat testis, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - [KO Validated] MLKL Rabbit pAb (A13451) }

    Immunohistochemistry - [KO Validated] MLKL Rabbit pAb (A13451)

    Immunohistochemistry of paraffin-embedded human breast cancer using MLKL antibody (A13451) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - [KO Validated] MLKL Rabbit pAb (A13451) }

    Immunohistochemistry - [KO Validated] MLKL Rabbit pAb (A13451)

    Immunohistochemistry of paraffin-embedded human liver cancer using MLKL Rabbit pAb (A13451) at dilution of 1:200 (40x lens).

    * For research use only. Not for therapeutic or diagnostic purposes.

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