Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MLKL Rabbit pAb (A13451)

Publications (2) Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MLKL Rabbit pAb (A13451)

Western blot analysis of extracts of various cell lines, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - MLKL Rabbit pAb (A13451)

Western blot analysis of extracts of Mouse liver, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - MLKL Rabbit pAb (A13451)

Western blot analysis of extracts of Rat testis, using MLKL antibody (A13451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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    Overview

    Product nameMLKL Rabbit pAb
    Catalog No.A13451
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-62 of mouse MLKL (Q9D2Y4).
    SequenceMDKLGQIIKLGQLIYEQCEKMKYCRKQCQRLGNRVHGLLQPLQRLQAQGKKNLPDDITAALG
    Gene ID74568
    Swiss protQ9D2Y4
    Synonyms9130019I15Rik
    Calculated MW54kDa
    Observed MW54kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:2000
    • IHC-P 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunohistochemistry    
    Positive samplesJurkat, NCI-H460, Mouse liver, Rat testis
    Cellular locationCell membrane, Cytoplasm
    Customer validation

    WB,IF(Rattus norvegicus)

    WB(Homo sapiens)

    MLKL Rabbit pAb images

    ABclonal:Western blot - MLKL Rabbit pAb (A13451)}

    Western blot - MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts of various cell lines, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - MLKL Rabbit pAb (A13451)}

    Western blot - MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts of Mouse liver, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - MLKL Rabbit pAb (A13451)}

    Western blot - MLKL Rabbit pAb (A13451)

    Western blot analysis of extracts of Rat testis, using MLKL antibody (A13451) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on Mlkl. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to Mlkl. (Distance between topics and target gene indicate popularity.) Mlkl

    * Data provided by citexs.com, for reference only.

    Publishing research using A13451? Please let us know so that we can cite the reference in this datasheet.

    Antibodies (5)

    ELISA Kits (1)

    Secondary Antibodies (22)

    * For research use only. Not for therapeutic or diagnostic purposes.

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