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[KO Validated] MLKL Rabbit mAb (A19685)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] MLKL Rabbit mAb (A19685)

Western blot analysis of extracts from normal (control) and MLKL knockout (KO) HeLa cells, using MLKL antibody (A19685) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] MLKL Rabbit mAb (A19685)

Western blot analysis of extracts of various cell lines, using MLKL antibody (A19685) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] MLKL Rabbit mAb (A19685)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] MLKL Rabbit mAb (A19685) at dilution of 1:100 (40x lens).

Overview

Product name[KO Validated] MLKL Rabbit mAb
Catalog No.A19685
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
ImmunogenA synthesized peptide derived from human MLKL.
SequenceEmail for sequence
Gene ID197259
Swiss protQ8NB16
SynonymshMLKL; MLKL
Calculated MW54kDa
Observed MW54KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNCI-H460, Jurkat, HeLa
Cellular locationCell membrane, Cytoplasm
Customer validation

WB(Gallus gallus)

    ABclonal:Western blot - [KO Validated] MLKL Rabbit mAb (A19685) }

    Western blot - [KO Validated] MLKL Rabbit mAb (A19685)

    Western blot analysis of extracts from normal (control) and MLKL knockout (KO) HeLa cells, using MLKL antibody (A19685) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - [KO Validated] MLKL Rabbit mAb (A19685) }

    Western blot - [KO Validated] MLKL Rabbit mAb (A19685)

    Western blot analysis of extracts of various cell lines, using MLKL antibody (A19685) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - [KO Validated] MLKL Rabbit mAb (A19685) }

    Immunohistochemistry - [KO Validated] MLKL Rabbit mAb (A19685)

    Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] MLKL Rabbit mAb (A19685) at dilution of 1:100 (40x lens).

    * For research use only. Not for therapeutic or diagnostic purposes.

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