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[KO Validated] JNK2 Rabbit pAb (A1251)

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ABclonal:Western blot - [KO Validated] JNK2 Rabbit pAb (A1251)
ABclonal:Western blot - [KO Validated] JNK2 Rabbit pAb (A1251)
ABclonal:Immunohistochemistry - [KO Validated] JNK2 Rabbit pAb (A1251)
ABclonal:Immunohistochemistry - [KO Validated] JNK2 Rabbit pAb (A1251)
ABclonal:Immunofluorescence - [KO Validated] JNK2 Rabbit pAb (A1251)
Review
ABclonal: review for [KO Validated] JNK2 Rabbit pAb(A1251)

Overview

Product name[KO Validated] JNK2 Rabbit pAb
Catalog No.A1251
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human JNK2 (NP_002743.3).
SequenceMSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR
Gene ID5601
Swiss protP45984
SynonymsMAPK9; JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; PRKM9; SAPK; SAPK1a; p54a; p54aSAPK
Calculated MW27kDa/44kDa/48kDa
Observed MW54kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF-7, DU145, THP-1, Mouse liver
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse heart tissue)

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] JNK2 Rabbit pAb (A1251) }

Western blot - [KO Validated] JNK2 Rabbit pAb (A1251)

Western blot analysis of extracts of various cell lines, using JNK2 antibody (A1251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] JNK2 Rabbit pAb (A1251) }

Western blot - [KO Validated] JNK2 Rabbit pAb (A1251)

Western blot analysis of extracts from normal (control) and JNK2 knockout (KO) 293T cells, using JNK2 antibody (A1251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] JNK2 Rabbit pAb (A1251) }

Immunohistochemistry - [KO Validated] JNK2 Rabbit pAb (A1251)

Immunohistochemistry of paraffin-embedded human breast cancer using JNK2 antibody (A1251) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] JNK2 Rabbit pAb (A1251) }

Immunohistochemistry - [KO Validated] JNK2 Rabbit pAb (A1251)

Immunohistochemistry of paraffin-embedded mouse brain using JNK2 antibody (A1251) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] JNK2 Rabbit pAb (A1251) }

Immunofluorescence - [KO Validated] JNK2 Rabbit pAb (A1251)

Immunofluorescence analysis of U2OS cells using JNK2 antibody (A1251). Blue: DAPI for nuclear staining.

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