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[KO Validated] MAPK6 Rabbit pAb (A15304)

Datasheet

ABclonal:Western blot - [KO Validated] MAPK6 Polyclonal Antibody (A15304)
ABclonal:Western blot - [KO Validated] MAPK6 Polyclonal Antibody (A15304)
ABclonal:Immunofluorescence - [KO Validated] MAPK6 Polyclonal Antibody (A15304)

Overview

Product name[KO Validated] MAPK6 Rabbit pAb
Catalog No.A15304
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).
SequenceNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYSDHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKKGKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVDKLNDLNSSVSQLELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFINQFCEVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
Gene ID5597
Swiss protQ16659
SynonymsMAPK6; ERK3; HsT17250; PRKM6; p97MAPK
Calculated MW82kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, A549, LO2, BxPC-3, 293T, Mouse brain
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - [KO Validated] MAPK6 Polyclonal Antibody (A15304) }

Western blot - [KO Validated] MAPK6 Polyclonal Antibody (A15304)

Western blot analysis of extracts of various cell lines, using MAPK6 antibody (A15304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Western blot - [KO Validated] MAPK6 Polyclonal Antibody (A15304) }

Western blot - [KO Validated] MAPK6 Polyclonal Antibody (A15304)

Western blot analysis of extracts from normal (control) and MAPK6 knockout (KO) HeLa cells, using MAPK6 antibody (A15304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - [KO Validated] MAPK6 Polyclonal Antibody (A15304) }

Immunofluorescence - [KO Validated] MAPK6 Polyclonal Antibody (A15304)

Immunofluorescence analysis of L929 cells using MAPK6 Polyclonal Antibody (A15304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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