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[KO Validated] MAP2K4 Rabbit pAb (A14781)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (A14781)
ABclonal:Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (A14781)

Overview

Product name[KO Validated] MAP2K4 Rabbit pAb
Catalog No.A14781
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).
SequenceLATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD
Gene ID6416
Swiss protP45985
SynonymsMAP2K4; JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1
Calculated MW44kDa/45kDa
Observed MW44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, BT-474, MCF-7, mouse thymus, mouse liver, rat liver
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (A14781) }

Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (A14781)

Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (A14781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (A14781) }

Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (A14781)

Western blot analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MAP2K4 antibody (A14781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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